aflatoxin contamination occurrence in milk and feed in kermanshah dairy farms by elisa technique

the aim of this study was to evaluate aflatoxin contamination in feed and milk in dairy farm in kermanshah. alfalfa, corn silage and milk samples from 9 industrial and semi industrial dairy farms were collected during 2 months. blood samples were collected via tail venipuncture from 10 % of dairy cows in each farm for biochemical and hematological parameters. aflatoxin contamination in feeds and milk samples was detected by elisa technique. aflatoxin contamination in alfalfa samples of small, medium and big dairy farm were: 12.4, 10.3 and 11.2microgram per kilogram respectively. there was no significant difference between contaminated alfalfa samples in dairy farms (p>0/05), however a significant difference was observed by time in contaminated alfalfa samples (p<0.01). the mean aflatoxin m1 in milk samples were 37.8, 29.7 and 34.9 nanogram/liter respectively. in this study the aflatoxin concentrations (afm1) in 33 % of the milk samples of dairy farm in kermanshah were greater than the maximum tolerance limit or about of european regulation (50 ppt) and in 67 % of the milk samples were below the limit of eu regulation.